Applications
Proteomics with nanoLC-MS
Nano-LC/MS of attomole digested Glutamate Dehydrogenase (GDG) with the polypropylene nanospray nozzle
Mobile Phases for LC: the mobile phases:
A: H20 + 0.5% Acetic Acid
B: Methanol + 0.5% Acetic Acid
The run was as follows:
- 10%B for 15 minutes (sample loading and column washing),
- 10%-90%B gradient for 20 minutes
- 90% B for 15 minutes
- 2 minute gradient back to 10%B
- Followed by a 10 minute re-equilibration

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Data were collected over the entire 1-hour run, with the first approximately 40 minutes of the run containing useful data.
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Results of nano-LC/MS of digested
Glutamate Dehydrogenase (GDG)
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